Characterization and Pathogenicity of Mycogone perniciosa Infecting Button Mushroom (Agaricus bisporus) and Bioefficacy of Plant Extracts Against the Pathogen
DOI:
https://doi.org/10.70884/mjsir.v86i1.610Keywords:
Aleuriospores, Fruiting bodies, Inhibition zone, Phialospores, Soil casing, Sporophore, Wet bubbleAbstract
Wet bubble disease represents a significant challenge in button mushroom cultivation. This study was conducted to verify the identity of the pathogen through morpho-cultural characterization and pathogenicity testing; and to assess the bioefficacy of plant extracts against the pathogen through in vitro agar disk diffusion assay. Infected button mushrooms were collected, isolated, and characterized, which confirmed its identity as Mycogone perniciosa. The pathogen produced white to light brown, fluffy mycelium with concentric rings; with hyaline, cylindrical phialospores and brown-pigmented, ellipsoid to globose aleuriospores. Pathogenicity tests demonstrated that a spore suspension (1.3 x 10^6 spores/mL) inoculated onto Agaricus bisporus fruiting bodies caused cap spotting and metallic brown staining within three days, which subsequently developed into white, cottony mycelial growth leading to external and internal decay by the seventh day. Furthermore, results of antifungal activity of eight plant extracts against M. perniciosa indicated that a 50 mg/mL concentration of tarragon exhibited the highest inhibitory activity, comparable to that of benomyl, from the third day post-application but diminished by the seventh day. Rosemary also demonstrated an inhibitory effect but only during the initial 3 days. Other plant extracts, including peppermint, oregano, ginger, lantana, neem, and angel’s trumpet, showed only short-term effects.
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