Mycelial growth of Scleroderma sp. as Affected by Culture Media

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Winslet J. Ramos Bernard S. Tad-awan


The prevailing problem towards commercial cultivation of ectomycorrhizal mushrooms including Scleroderma sp. is the lack of protocol and information on the culture medium to produce inoculant, which is required in producing the mushroom alongside with pine. Scleroderma can be tapped as an inoculant for pine trees to increase the production of Scleroderma at the mountains which not only helps the pine trees to absorb water and nutrients from the soil but also accelerates the shoot growth and rehabilitates degraded land. To attain this, a protocol needs to be developed along with the appropriate culture medium process that may result in the mass production of Scleroderma mycelia to serve as inoculant for pine seedlings resulting in increased pine areas and production of Scleroderma. Thus, a study to compare the mycelial growth of Scleroderma sp. on two kinds of media was conducted. Results revealed that the use of Murashige and Skoog medium promoted faster mycelial growth and had the heaviest mycelial biomass. It is recommended that further studies be conducted on the optimal conditions of Scleroderma sp. as well as its inoculation to pine seedlings and testing the same media on other ectomycorrhizal fungi to strengthen and validate the study’s method as protocol on culture medium preparation.

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